资源类型

期刊论文 182

会议视频 6

年份

2024 2

2023 18

2022 27

2021 21

2020 15

2019 15

2018 8

2017 17

2016 12

2015 8

2014 7

2013 1

2012 2

2011 6

2010 9

2009 2

2008 3

2007 7

2006 1

2005 1

展开 ︾

关键词

抗生素 3

3D打印 2

绿色化工 2

2-羟基丁酸 1

3D生物打印 1

Au/Ti双功能催化剂 1

H2有效利用率 1

HBV相关疾病 1

HVAC系统 1

Nd-YAG 1

RE-Si3N4 1

T-S模糊模型 1

hsa-miR-197 1

丙烯环氧化 1

中国微生物组计划 1

主–客体络合 1

乏氧 1

亚甲基蓝 1

人脑功能磁共振 1

展开 ︾

检索范围：

排序： 展示方式：

Microbial community and functional genes in the rhizosphere of alfalfa in crude oil-contaminated soil

Yi ZHONG, Jian WANG, Yizhi SONG, Yuting LIANG, Guanghe LI

《环境科学与工程前沿（英文）》 2012年 第6卷 第6期   页码 797-805 doi: 10.1007/s11783-012-0405-z

摘要： A rhizobox system constructed with crude oil-contaminated soil was vegetated with alfalfa ( L.) to evaluate the rhizosphere effects on the soil microbial population and functional structure, and to explore the potential mechanisms by which plants enhance the removal of crude oil in soil. During the 80-day experiment, 31.6% of oil was removed from the adjacent rhizosphere (AR); this value was 27% and 53% higher than the percentage of oil removed from the far rhizosphere (FR) and from the non-rhizosphere (NR), respectively. The populations of heterotrophic bacteria and hydrocarbon-degrading bacteria were higher in the AR and FR than in the NR. However, the removal rate of crude oil was positively correlated with the proportion of hydrocarbon-degrading bacteria in the rhizosphere. In total, 796, 731, and 379 functional genes were detected by microarray in the AR, FR, and NR, respectively. Higher proportions of functional genes related to carbon degradation and organic remediation, were found in rhizosphere soil compared with NR soil, suggesting that the rhizosphere selectively increased the abundance of these specific functional genes. The increase in water-holding capacity and decrease in pH as well as salinity of the soil all followed the order of AR>FR>NR. Canonical component analysis showed that salinity was the most important environmental factor influencing the microbial functional structure in the rhizosphere and that salinity was negatively correlated with the abundance of carbon and organic degradation genes.

关键词： crude oil-contaminated soil     phytoremediation     rhizosphere effects     rhizobox     functional genes    

HTML PDF 收藏

Immunotherapeutic effects on murine pancreatic carcinoma by β-elemene combined with dendritic cells modified with genes

TAN Guang, WANG Zhongyu, CHE Luanqing, YIN Shuo

《医学前沿（英文）》 2007年 第1卷 第1期   页码 41-45 doi: 10.1007/s11684-007-0008-4

摘要： The dendritic cell vaccine is a treatment vaccine with potent clinical applications. Functional cytokines can enhance dendritic cell anti-tumor immune responses. This experiment was conducted to study the effects of bone marrow-deriv

关键词： Functional     experiment     anti-tumor     dendritic     clinical    

HTML PDF 收藏

Characterization of the genes involved in nitrogen cycling in wastewater treatment plants using DNA microarray

Junqin PANG, Masami MATSUDA, Masashi KURODA, Daisuke INOUE, Kazunari SEI, Kei NISHIDA, Michihiko IKE

《环境科学与工程前沿（英文）》 2016年 第10卷 第4期 doi: 10.1007/s11783-016-0846-x

摘要： Nitrogen-cycling microbial communities in municipal WWTPs were characterized. Numbers of , and genes were quantified by MPN-PCR. Diversity of whole nitrogen-cycling communities was analyzed with DNA microarray. CAS process retained diverse nitrogen cycling populations. Specific, limited populations may be dominated in nitrogen removal processes. To improve nitrogen removal performance of wastewater treatment plants (WWTPs), it is essential to understand the behavior of nitrogen cycling communities, which comprise various microorganisms. This study characterized the quantity and diversity of nitrogen cycling genes in various processes of municipal WWTPs by employing two molecular-based methods:most probable number-polymerase chain reaction (MPN-PCR) and DNA microarray. MPN-PCR analysis revealed that gene quantities were not statistically different among processes, suggesting that conventional activated sludge processes (CAS) are similar to nitrogen removal processes in their ability to retain an adequate population of nitrogen cycling microorganisms. Furthermore, most processes in the WWTPs that were researched shared a pattern:the and the bacterial genes were more abundant than the and archaeal genes, respectively. DNA microarray analysis revealed that several kinds of nitrification and denitrification genes were detected in both CAS and anaerobic-oxic processes (AO), whereas limited genes were detected in nitrogen removal processes. Results of this study suggest that CAS maintains a diverse community of nitrogen cycling microorganisms; moreover, the microbial communities in nitrogen removal processes may be specific.

关键词： DNA microarray analysis     Nitrogen cycling functional genes     Most probable number-polymerase chain reaction (MPN-PCR)     Wastewater treatment plants (WWTPs)    

HTML PDF 收藏

Metagenomic analysis on resistance genes in water and microplastics from a mariculture system

《环境科学与工程前沿（英文）》 2022年 第16卷 第1期   页码 4-4 doi: 10.1007/s11783-021-1438-y

摘要：

Microplastics existing widely in different matrices have been regarded as a reservoir for emerging contaminants. Mariculture systems have been observed to host microplastics and antibiotic resistance genes (ARGs). However, more information on proliferation of ARGs and metal resistance genes (MRGs) in mariculture system at the presence of microplastics is needed.

关键词： Antibiotic resistance genes     Metal resistance genes     Metagenomic analysis     Microplastics     Mariculture    

HTML PDF 收藏

Distribution and removal of antibiotic resistance genes during anaerobic sludge digestion with alkaline

Mengli Wang, Ruying Li, Qing Zhao

《环境科学与工程前沿（英文）》 2019年 第13卷 第3期 doi: 10.1007/s11783-019-1127-2

摘要：

Sludge digestion is critical to control the spread of ARGs from wastewater to soil.

Fate of ARGs in three pretreatment-AD processes was investigated.

UP was more efficient for ARGs removal than AP and THP in pretreatment-AD process.

The total ARGs concentration showed significant correlation with 16S rRNA gene.

The bacteria carrying ARGs could be mainly affiliated with Proteobacteria.

关键词： Tetracycline resistance genes     Sulfonamide resistance genes     16S rRNA gene     Sludge pretreatment     Anaerobic sludge digestion    

HTML PDF 收藏

Environmental effects and risk control of antibiotic resistance genes in the organic solid waste aerobic

《环境科学与工程前沿（英文）》 2021年 第15卷 第6期 doi: 10.1007/s11783-021-1415-5

摘要：

• ARGs were detected in livestock manure, sludge, food waste and fermentation dregs.

关键词： Antibiotic resistance genes     Organic solid waste     Aerobic composting     Livestock manure     Sludge    

HTML PDF 收藏

ultraviolet/chlorine process on microbial community structure, typical pathogens, and antibiotic resistance genes

《环境科学与工程前沿（英文）》 2022年 第16卷 第8期 doi: 10.1007/s11783-022-1521-z

摘要：

• UV/chlorine can effectively remove VBNC pathogens, ARGs and MGEs in reclaimed water.

关键词： UV/chlorine process     Pathogen     Antibiotic resistance genes     High-throughput qPCR     Reclaimed water    

HTML PDF 收藏

Distribution of antibiotic resistance genes and their association with bacteria and viruses in decentralized

《环境科学与工程前沿（英文）》 2022年 第16卷 第3期 doi: 10.1007/s11783-021-1469-4

摘要：

• Distribution of ARGs in decentralized sewage facilities were investigated.

关键词： Decentralized sewage treatment facilities     Antibiotic resistance genes     Virus     Metagenomics     Network analysis    

HTML PDF 收藏

Development and applications of functional gene microarrays in the analysis of the functional diversity

Zhili HE, Joy D. VAN NOSTRAND, Ye DENG, Jizhong ZHOU

《环境科学与工程前沿（英文）》 2011年 第5卷 第1期   页码 1-20 doi: 10.1007/s11783-011-0301-y

摘要： Functional gene arrays (FGAs) are a special type of microarrays containing probes for key genes involved in microbial functional processes, such as biogeochemical cycling of carbon, nitrogen, sulfur, phosphorus, and metals, biodegradation of environmental contaminants, energy processing, and stress responses. GeoChips are considered as the most comprehensive FGAs. Experimentally established probe design criteria and a computational pipeline integrating sequence retrieval, probe design and verification, array construction, data analysis, and automatic update are used to develop the GeoChip technology. GeoChip has been systematically evaluated and demonstrated to be a powerful tool for rapid, specific, sensitive, and quantitative analysis of microbial communities in a high-throughput manner. Several generations of GeoChip have been developed and applied to investigate the functional diversity, composition, structure, function, and dynamics of a variety of microbial communities from different habitats, such as water, soil, marine, bioreactor, human microbiome, and extreme ecosystems. GeoChip is able to address fundamental questions related to global change, bioenergy, bioremediation, agricultural operation, land use, human health, environmental restoration, and ecological theories and to link the microbial community structure to environmental factors and ecosystem functioning.

关键词： functional gene arrays (FGAs)     GeoChip     microbial communities     functional diversity/composition/structure     environmental factor     ecosystem functioning    

HTML PDF 收藏

Research progress in pathogenic genes of hereditary non-syndromic mid-frequency deafness

null

《医学前沿（英文）》 2016年 第10卷 第2期   页码 137-142 doi: 10.1007/s11684-016-0449-8

摘要：

Hearing impairment is considered as the most prevalent impairment worldwide. Almost 600 million people in the world suffer from mild or moderate hearing impairment, an estimated 10% of the human population. Genetic factors play an important role in the pathogenesis of this disorder. Hereditary hearing loss is divided into syndromic hearing loss (associated with other anomalies) and non-syndromic hearing loss (not associated with other anomalies). Approximately 80% of genetic deafness is non-syndromic. On the basis of the frequency of hearing loss, hereditary non-syndromic hearing loss can be divided into high-, mid-, low-, and total-frequency hearing loss. An audiometric finding of mid-frequency sensorineural hearing loss, or a “bowl-shaped” audiogram, is uncommon. Up to now, merely 7 loci have been linked to mid-frequency hearing loss. Only four genetic mid-frequency deafness genes, namely, DFNA10 (EYA4), DFNA8/12 (TECTA), DFNA13 (COL11A2), DFNA44 (CCDC50), have been reported to date. This review summarizes the research progress of the four genes to draw attention to mid-frequency deafness genes.

关键词： hereditary non-syndromic hearing loss     mid-frequency hearing loss     deafness genes    

HTML PDF 收藏

Antibiotic resistance genes in manure-amended paddy soils across eastern China: Occurrence and influencing

《环境科学与工程前沿（英文）》 2022年 第16卷 第7期 doi: 10.1007/s11783-021-1499-y

摘要：

• Manure fertilization resulted in antibiotic residues and increased metal contents.

关键词： Pig manure     Antibiotics     Metals     Antibiotic resistance genes     Paddy fields    

HTML PDF 收藏

Effects of dwarfing genes on water use efficiency of bread wheat

Jiakun YAN, Suiqi ZHANG

《农业科学与工程前沿（英文）》 2017年 第4卷 第2期   页码 126-134 doi: 10.15302/J-FASE-2017134

摘要： Climate change has increased the risk of drought, which significantly limits plant productivity. Various ways of increasing water availability and sustaining growth of crop plants in drought-prone environments are available. Genetic advances in grain yields under rainfed conditions have been achieved with the introduction of dwarfing genes. A thorough understanding of the effects of different dwarfing genes on root growth, coleoptile length, grain yields and water using efficiency (WUE) will provide opportunities to select appropriate genes for breeding high WUE and grain yield cultivars. This review focuses on the mechanism involved in genes that reduce plant height and affect root and coleoptile length, their consequent effects on grain yields and WUE, and suggests that for rainfed and irrigation-limited environments, combining GAR and GAI dwarfing genes in breeding may help boost WUE and yields, and more materials from different parental sources should be collected to assess opportunities for potential comprehensive application of specific genes.

关键词： coleoptile length     wheat     dwarfing genes     grain yield     root     water use efficiency    

HTML PDF 收藏

Degradation of extracellular genomic, plasmid DNA and specific antibiotic resistance genes by chlorination

Menglu Zhang, Sheng Chen, Xin Yu, Peter Vikesland, Amy Pruden

《环境科学与工程前沿（英文）》 2019年 第13卷 第3期 doi: 10.1007/s11783-019-1124-5

摘要：

Extracellular DNA structure damaged by chlorination was characterized.

Integrity of extracellular ARG genetic information after chlorination was determined.

Typical chlorine doses will likely effectively diminish extracellular DNA and ARGs.

Plasmid DNA/ARGs were less readily broken down than genomic DNA.

The Bioanalyzer methodology effectively documented damage incurred to DNA.

关键词： Antibiotic resistance     Antibiotic resistance genes (ARGs)     Extracellular DNA/ARGs     Chlorination    

HTML PDF 收藏

Design of tandem genes cluster for isoflavone engineering

Xunli XIA PhD , Guangxiao YANG PhD , Guangyuan HE PhD ,

《医学前沿（英文）》 2009年 第3卷 第3期   页码 292-296 doi: 10.1007/s11684-009-0057-y

摘要： In this paper, a universal effective novel method of constructing tandem genes cluster for isoflavone engineering was reported. A tandem genes cluster () of secondary metabolites of plant isoflavones was constructed by using the chalcone synthase (CHS), chalcone isomerase (CHI), and isoflavone synthase (IFS) (GenBank accession numbers EU526827, EU526829, EU526830) in only one recombination with the pET22b vector. The resulting expression vector pET- was heterogeneously expressed and co-incubated with barrenwort extractions, and the genistein-like component was detected.

关键词： isoflavones engineering     secondary metabolism     tandem genes cluster    

HTML PDF 收藏

with sulfamethoxazole and norfloxacin: Dynamics of microbial communities and evolution of resistance genes

《环境科学与工程前沿（英文）》 2021年 第15卷 第5期 doi: 10.1007/s11783-020-1342-x

摘要：

• SMX addition had negative effect on acetoclastic methanogens in mesophilic AD.

关键词： Pig manure     Antibiotics     Anaerobic digestion     Resistance genes     Microbial community    

HTML PDF 收藏

标题 作者 时间 类型 操作

Microbial community and functional genes in the rhizosphere of alfalfa in crude oil-contaminated soil

Yi ZHONG, Jian WANG, Yizhi SONG, Yuting LIANG, Guanghe LI

期刊论文

Immunotherapeutic effects on murine pancreatic carcinoma by β-elemene combined with dendritic cells modified with genes

TAN Guang, WANG Zhongyu, CHE Luanqing, YIN Shuo

期刊论文

Characterization of the genes involved in nitrogen cycling in wastewater treatment plants using DNA microarray

Junqin PANG, Masami MATSUDA, Masashi KURODA, Daisuke INOUE, Kazunari SEI, Kei NISHIDA, Michihiko IKE

期刊论文

Metagenomic analysis on resistance genes in water and microplastics from a mariculture system

期刊论文

Distribution and removal of antibiotic resistance genes during anaerobic sludge digestion with alkaline

Mengli Wang, Ruying Li, Qing Zhao

期刊论文

Environmental effects and risk control of antibiotic resistance genes in the organic solid waste aerobic

期刊论文

ultraviolet/chlorine process on microbial community structure, typical pathogens, and antibiotic resistance genes

期刊论文

Distribution of antibiotic resistance genes and their association with bacteria and viruses in decentralized

期刊论文

Development and applications of functional gene microarrays in the analysis of the functional diversity

Zhili HE, Joy D. VAN NOSTRAND, Ye DENG, Jizhong ZHOU

期刊论文

Research progress in pathogenic genes of hereditary non-syndromic mid-frequency deafness

null

期刊论文

Antibiotic resistance genes in manure-amended paddy soils across eastern China: Occurrence and influencing

期刊论文

Effects of dwarfing genes on water use efficiency of bread wheat

Jiakun YAN, Suiqi ZHANG

期刊论文

Degradation of extracellular genomic, plasmid DNA and specific antibiotic resistance genes by chlorination

Menglu Zhang, Sheng Chen, Xin Yu, Peter Vikesland, Amy Pruden

期刊论文

Design of tandem genes cluster for isoflavone engineering

Xunli XIA PhD , Guangxiao YANG PhD , Guangyuan HE PhD ,

期刊论文

with sulfamethoxazole and norfloxacin: Dynamics of microbial communities and evolution of resistance genes

期刊论文